CD43 monoclonal antibody, clone DF-T1
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Specification
Product Description
Mouse monoclonal antibody raised against CD43 (SPN).
Immunogen
Myeloblastic KG1 cells.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG1, kappa
Recommend Usage
Flow Cytometry (0.5-2 ug/106 cells)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% BSA, 0.05% sodium azide)
Storage Instruction
Store at 4°C.
Avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) analysis of human spleen with CD43 monoclonal antibody, clone DF-T1 (Cat # MAB11297) at 1:200 using peroxidase-conjugate and DAB chromogen.Flow Cytometry
Flow cytometric analysis of lymphocyte gated population of PBMC using either CD43 monoclonal antibody, clone DF-T1 (Cat # MAB11297) (0.5ug/106 cells) or isotype control following surface staining protocol. PE-conjugated goat anti-mouse IgG secondary antibody is used to identify the positive staining on PPI negative lymphocyte gated population. -
Gene Info — SPN
Entrez GeneID
6693Gene Name
SPN
Gene Alias
CD43, GPL115, LSN
Gene Description
sialophorin
Omim ID
182160Gene Ontology
HyperlinkGene Summary
Sialophorin (leukosialin) is a major sialoglycoprotein on the surface of human T lymphocytes, monocytes, granulocytes, and some B lymphocytes, which appears to be important for immune function and may be part of a physiologic ligand-receptor complex involved in T-cell activation.[supplied by OMIM
Other Designations
leukosialin|sialophorin (gpL115, leukosialin, CD43)|sialophorin (leukosialin, CD43)
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Interactome
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