Immunohistochemistry (Formalin-fixed paraffin-embedded sections) analysis of human spleen with CD43 monoclonal antibody, clone DF-T1 (Cat # MAB11297) at 1:200 using peroxidase-conjugate and DAB chromogen.
Flow cytometric analysis of lymphocyte gated population of PBMC using either CD43 monoclonal antibody, clone DF-T1 (Cat # MAB11297) (0.5ug/106 cells) or isotype control following surface staining protocol. PE-conjugated goat anti-mouse IgG secondary antibody is used to identify the positive staining on PPI negative lymphocyte gated population.
Sialophorin (leukosialin) is a major sialoglycoprotein on the surface of human T lymphocytes, monocytes, granulocytes, and some B lymphocytes, which appears to be important for immune function and may be part of a physiologic ligand-receptor complex involved in T-cell activation.[supplied by OMIM