Mouse monoclonal antibody raised against CD35 (CR1).
Intact human monocytes (Hogg et al, 1984).
Protein G purification
Western Blot (1:100-1:2000) Flow Cytometry (1:10-1:1000) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:400) Immunoprecipitation (1:10-1:500) The optimal working dilution should be determined by the end user.
In PBS (0.05% BSA, 0.05% sodium azide)
Store at 4°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Immunohistochemistry (Formalin-fixed paraffin-embedded sections) analysis of human tonsil with CD35 monoclonal antibody, clone E11 (Cat # MAB11296) at 1:200 using peroxidase-conjugate and DAB chromogen (Note: membrane staining).
This gene is a member of the receptors of complement activation (RCA) family and is located in the 'cluster RCA' region of chromosome 1. The gene encodes a monomeric single-pass type I membrane glycoprotein found on erythrocytes, leukocytes, glomerular podocytes, and splenic follicular dendritic cells. The Knops blood group system is a system of antigens located on this protein. The protein mediates cellular binding to particles and immune complexes that have activated complement. Decreases in expression of this protein and/or mutations in its gene have been associated with gallbladder carcinomas, mesangiocapillary glomerulonephritis, systemic lupus erythematosus and sarcoidosis. Mutations in this gene have also been associated with a reduction in Plasmodium falciparum rosetting, conferring protection against severe malaria. Alternate allele-specific splice variants, encoding different isoforms, have been characterized. Additional allele specific isoforms, including a secreted form, have been described but have not been fully characterized. [provided by RefSeq