Mouse monoclonal antibody raised against synthetic peptide of BCL2.
A synthetic peptide corresponding to amino acids 41-54 (GAAPAPGIFSSQPG-Cys) of human BCL2 (Pezzella et al, 1990).
Protein G purification
Western Blot (1-3 ug/ml) Flow Cytometry (0.5 ug/l06 cells) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:250-1:1500) Immunoprecipitation (1:10 - 1:500) The optimal working dilution should be determined by the end user.
In PBS (0.05% BSA, 0.05% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Immunohistochemistry (Formalin-fixed paraffin-embedded sections) analysis of non-Hodgkin's lymphoma with BCL2 monoclonal antibody, clone 100/D5 (Cat # MAB11293) at 1:500 using peroxidase-conjugate and DAB chromogen (Note: cell membrane and cytoplasmic staining).
Flow cytometric analysis of Jurkat cells using BCL2 monoclonal antibody, clone 100/D5 (Cat # MAB11293) (red) and isotype control (green) at 0.5 ug/106 cells.
This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends. [provided by RefSeq