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IGL@ monoclonal antibody, clone LcN-2 

  • Catalog # : MAB11290
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against IGL@.
  • Immunogen:
  • Purified human IgG.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • ~22.5
  • Reactivity:
  • Human
  • Specificity:
  • This MAb is specific to lambda light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains.
  • Form:
  • Liquid
  • Purification:
  • Protein A/G purification
  • Isotype:
  • IgG2a, kappa
  • Recommend Usage:
  • Flow Cytometry (1-2 ug/million cells)
    Immunofluorescence (1-2 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.25-0.5 ug/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
    Western Blot (1-2 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 10 mM PBS (0.05% BSA, 0.05% sodium azide).
  • Storage Instruction:
  • Store at 4°C.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • Western blot analysis of human intestine using IGL@ monoclonal antibody, clone LcN-2 (Cat # MAB11290) at 2 ug/mL.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human tonsil with IGL@ monoclonal antibody, clone LcN-2 (Cat # MAB11290) at 1:200 using peroxidase-conjugate and DAB chromogen.
  • Immunofluorescence
  • Immunofluorescence
  • Confocal Immunofluorescent analysis of Ramos cells (A) using AF488-labeled IGL@ monoclonal antibody, clone LcN-2 (Cat # MAB11290) (Green). DAPI was used to stain the cell nuclei (blue). (B) using AF488-labeled Isotype Control MAb (IgG2a) (Green). DAPI was used to stain the cell nuclei (blue). (Negative Control).
  • Flow Cytometry
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Flow Cytometry
  • Gene Information
  • Entrez GeneID:
  • 3535
  • Gene Name:
  • IGL@
  • Gene Alias:
  • IGL,MGC88804
  • Gene Description:
  • immunoglobulin lambda locus
  • Gene Summary:
  • Immunoglobulins recognize foreign antigens and initiate immune responses such as phagocytosis and the complement system. Each immunoglobulin molecule consists of two identical heavy chains and two identical light chains. There are two classes of light chains, kappa and lambda. This region represents the germline organization of the lambda light chain locus. The locus includes V (variable), J (joining), and C (constant) segments. During B cell development, a recombination event at the DNA level joins a single V segment with a J segment; the C segment is later joined by splicing at the RNA level. Recombination of many different V segments with several J segments provides a wide range of antigen recognition. Additional diversity is attained by junctional diversity, resulting from the random additional of nucleotides by terminal deoxynucleotidyltransferase, and by somatic hypermutation, which occurs during B cell maturation in the spleen and lymph nodes. Several V segments and three C segments are known to be incapable of encoding a protein and are considered pseudogenes. The locus also includes several non-immunoglobulin genes, many of which are pseudogenes or are predicted by automated computational analysis or homology to other species. [provided by RefSeq
  • Other Designations:
  • immunoglobulin lambda gene cluster
  • Gene Information
  • Entrez GeneID:
  • 3546
  • Gene Name:
  • IGLV@
  • Gene Alias:
  • IGLV
  • Gene Description:
  • immunoglobulin lambda variable group
  • Other Designations:
  • -
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