IGL@ monoclonal antibody, clone LcN-2
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of human intestine using IGL@ monoclonal antibody, clone LcN-2 (Cat # MAB11290) at 2 ug/mL.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human tonsil with IGL@ monoclonal antibody, clone LcN-2 (Cat # MAB11290) at 1:200 using peroxidase-conjugate and DAB chromogen.
Immunofluorescence
Confocal Immunofluorescent analysis of Ramos cells (A) using AF488-labeled IGL@ monoclonal antibody, clone LcN-2 (Cat # MAB11290) (Green). DAPI was used to stain the cell nuclei (blue). (B) using AF488-labeled Isotype Control MAb (IgG2a) (Green). DAPI was used to stain the cell nuclei (blue). (Negative Control).
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Specification
Product Description
Mouse monoclonal antibody raised against IGL@.
Immunogen
Purified human IgG.
Host
Mouse
Theoretical MW (kDa)
~22.5
Reactivity
Human
Specificity
This MAb is specific to lambda light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains.
Form
Liquid
Purification
Protein A/G purification
Isotype
IgG2a, kappa
Recommend Usage
Flow Cytometry (1-2 ug/million cells)
Immunofluorescence (1-2 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.25-0.5 ug/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Western Blot (1-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM PBS (0.05% BSA, 0.05% sodium azide).
Storage Instruction
Store at 4°C.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of human intestine using IGL@ monoclonal antibody, clone LcN-2 (Cat # MAB11290) at 2 ug/mL.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human tonsil with IGL@ monoclonal antibody, clone LcN-2 (Cat # MAB11290) at 1:200 using peroxidase-conjugate and DAB chromogen.Immunofluorescence
Confocal Immunofluorescent analysis of Ramos cells (A) using AF488-labeled IGL@ monoclonal antibody, clone LcN-2 (Cat # MAB11290) (Green). DAPI was used to stain the cell nuclei (blue). (B) using AF488-labeled Isotype Control MAb (IgG2a) (Green). DAPI was used to stain the cell nuclei (blue). (Negative Control).Flow Cytometry
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Gene Info — IGL@
Entrez GeneID
3535Gene Name
IGL@
Gene Alias
IGL, MGC88804
Gene Description
immunoglobulin lambda locus
Gene Ontology
HyperlinkGene Summary
Immunoglobulins recognize foreign antigens and initiate immune responses such as phagocytosis and the complement system. Each immunoglobulin molecule consists of two identical heavy chains and two identical light chains. There are two classes of light chains, kappa and lambda. This region represents the germline organization of the lambda light chain locus. The locus includes V (variable), J (joining), and C (constant) segments. During B cell development, a recombination event at the DNA level joins a single V segment with a J segment; the C segment is later joined by splicing at the RNA level. Recombination of many different V segments with several J segments provides a wide range of antigen recognition. Additional diversity is attained by junctional diversity, resulting from the random additional of nucleotides by terminal deoxynucleotidyltransferase, and by somatic hypermutation, which occurs during B cell maturation in the spleen and lymph nodes. Several V segments and three C segments are known to be incapable of encoding a protein and are considered pseudogenes. The locus also includes several non-immunoglobulin genes, many of which are pseudogenes or are predicted by automated computational analysis or homology to other species. [provided by RefSeq
Other Designations
immunoglobulin lambda gene cluster
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Gene Info — IGLV@
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Interactome
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Disease
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