S100 monoclonal antibody, clone 4C4.9
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Specification
Product Description
Mouse monoclonal antibody raised against S100.
Immunogen
S100 protein purified from bovine brain
Host
Mouse
Reactivity
Bovine, Human, Mouse, Rat
Form
Liquid
Purification
Protein G affinity chromatography
Isotype
IgG2a
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:400)
Western Blot (1-3 ug/ml)
The optimal working dilution should be determined by the end user.Storage Buffer
In 0.2 mg/ml PBS (0.05% BSA, 0.05% sodium azide)
Storage Instruction
Store at 4°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of human brain using S100 monoclonal antibody, clone 4C4.9 (Cat # MAB11286) at 1 ug/ml.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human melanoma with S100 monoclonal antibody, clone 4C4.9 (Cat # MAB11286) at 1:200 using peroxidase-conjugate and DAB chromogen.
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