Western blot analysis of cell lysates of LNCaP (20 ug) were resolved by SDS - PAGE , transferred to NC membrane and probed with FOLH1 monoclonal antibody , clone k2G3 (1 : 2000) (Cat # MAB1128). Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system.
Immunofluorescence staining of human LNCaP cell colony with FOLH1 monoclonal antibody , clone k2G3 (Cat # MAB1128) .
This gene encodes a type II transmembrane glycoprotein belonging to the M28 peptidase family. The protein acts as a glutamate carboxypeptidase on different alternative substrates, including the nutrient folate and the neuropeptide N-acetyl-l-aspartyl-l-glutamate and is expressed in a number of tissues such as prostate, central and peripheral nervous system and kidney. A mutation in this gene may be associated with impaired intestinal absorption of dietary folates, resulting in low blood folate levels and consequent hyperhomocysteinemia. Expression of this protein in the brain may be involved in a number of pathological conditions associated with glutamate excitotoxicity. In the prostate the protein is up-regulated in cancerous cells and is used as an effective diagnostic and prognostic indicator of prostate cancer. This gene likely arose from a duplication event of a nearby chromosomal region. Alternative splicing gives rise to multiple transcript variants. [provided by RefSeq
N-acetylated alpha-linked acidic dipeptidase 1,cell growth-inhibiting protein 27,folate hydrolase 1,folylpoly-gamma-glutamate carboxypeptidase,glutamate carboxylase II,glutamate carboxypeptidase II,membrane glutamate carboxypeptidase,prostate specific mem