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Last updated: 2016/12/4
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PODXL monoclonal antibody, clone 3D3

  • Catalog # : MAB11270
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against PODXL.
  • Immunogen:
  • A mixture of recombinant protein corresponding to intracellular, transmembrane, and extracellular domain of human PODXL.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • Flow Cytometry (1-2 ug/106 cells)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:500-1:1000)
    Western Blot (3-5 ug/ml)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.05% BSA, 0.05% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of HeLa cell lysates by PODXL monoclonal antibody, clone 3D3 (Cat # MAB11270) at 3 ug/ml.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining of human angiosarcoma (20X) with PODXL monoclonal antibody, clone 3D3 (Cat # MAB11270) at 1:500 dilution.
  • Immunofluorescence
  • Immunoprecipitation
  • Flow Cytometry
  • Flow Cytometry
  • Surface staining of NCCIT cells with PODXL monoclonal antibody, clone 3D3 (Cat # MAB11270) at 1 ug/106 cells.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunofluorescence
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 5420
  • Gene Name:
  • PODXL
  • Gene Alias:
  • Gp200,MGC138240,PC,PCLP
  • Gene Description:
  • podocalyxin-like
  • Gene Summary:
  • This gene encodes a member of the sialomucin protein family. The encoded protein was originally identified as an important component of glomerular podocytes. Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane. Other biological activities of the encoded protein include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin. [provided by RefSeq
  • Other Designations:
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