Western blot analysis of cell lysates of HeLa (30 ug) was resolved by SDS - PAGE , transferred to NC membrane and probed with HSF1 monoclonal antibody , clone J7F9 (1 : 1000) (Cat # MAB1126). Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system.
The product of this gene is a heat-shock transcription factor. Transcription of heat-shock genes is rapidly induced after temperature stress. Hsp90, by itself and/or associated with multichaperone complexes, is a major repressor of this gene. [provided by RefSeq