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Last updated: 2017/12/10
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HLA-G monoclonal antibody, clone 87G

  • Catalog # : MAB11223
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against HLA-G and B2M.
  • Immunogen:
  • HLA-B27 transgenic mice were imunized with H-2 identical murine cells transfected with and expressing genes encoding HLA-G and human beta2-microglobulin.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Specificity:
  • This antibody recognizes both membrane-bound and soluble forms of HLA-G (HLA-G1and HLA-G5). HLA-G belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of trophoblast cells. This antibody blocks interaction of HLA-G with inhibitory receptors.
  • Form:
  • Liquid
  • Purification:
  • Protein-A affinity chromatography
  • Purity:
  • > 95% by SDS-PAGE
  • Endotoxin Level:
  • < 0.01 EU/ug
  • Isotype:
  • IgG2a
  • Recommend Usage:
  • ELISA (1 ug/mL)
    Flow Cytometry (2 ug/ml)
    Immunohistochemistry (Frozen sections) (10 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4
  • Storage Instruction:
  • Store at 4°C. Do not freeze.
    Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Immunohistochemistry (Frozen sections)
  • ELISA
  • Functional Study
  • Functional Study
  • Analysis of cytolytical activity of human polyclonal NK cells on target melanoma cells. Blocking of HLA-G1 on transfectants with anti-human HLA-G (87G) restored specific lysis.
    Target cells : M8 cell line transfected with empty vector (column 1) and with HLA-G1 cDNA (columns 2-6).
    Blocking antibodies :
    Column 1-2 : none.
    Column 3 : Isotype mouse IgG2a control.
    Column 4 : HLA-G monoclonal antibody, clone 87G (Cat # MAB11223).
    Column 5 : HLA-G (Fab) monoclonal antibody, clone 87G.
    Column 6 : HLA-G monoclonal antibody, clone MEM-G/9 (Cat # MAB3855).
  • Flow Cytometry
  • Application Image
  • Immunohistochemistry (Frozen sections)
  • ELISA
  • Flow Cytometry
  • Gene Information
  • Entrez GeneID:
  • 3135
  • Gene Name:
  • HLA-G
  • Gene Alias:
  • MHC-G
  • Gene Description:
  • major histocompatibility complex, class I, G
  • Gene Summary:
  • HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail. [provided by RefSeq
  • Other Designations:
  • HLA class I molecule,HLA-G histocompatibility antigen, class I, G,MHC class I antigen,OTTHUMP00000039485,b2 microglobulin
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