Mouse monoclonal antibody raised against HLA-G and B2M.
HLA-B27 transgenic mice were imunized with H-2 identical murine cells transfected with and expressing genes encoding HLA-G and human beta2-microglobulin.
This antibody recognizes both membrane-bound and soluble forms of HLA-G (HLA-G1and HLA-G5). HLA-G belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of trophoblast cells. This antibody blocks interaction of HLA-G with inhibitory receptors.
Protein-A affinity chromatography
> 95% by SDS-PAGE
< 0.01 EU/ug
ELISA (1 ug/mL) Flow Cytometry (2 ug/ml) Immunohistochemistry (Frozen sections) (10 ug/mL) The optimal working dilution should be determined by the end user.
In PBS, pH 7.4
Store at 4°C. Do not freeze. Aliquot to avoid repeated freezing and thawing.
HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail. [provided by RefSeq
HLA class I molecule,HLA-G histocompatibility antigen, class I, G,MHC class I antigen,OTTHUMP00000039485,b2 microglobulin