MAFK monoclonal antibody, clone AT2F7
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Jurkat cell lysate (30 ug) by using MAFK monoclonal antibody, clone AT2F7 (Cat # MAB11212) (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant MAFK.
Immunogen
Recombinant protein corresponding to amino acids 1-156 of human MAFK.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Concentration
1 mg/mL
Isotype
IgG2a, kappa
Recommend Usage
ELISA
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Jurkat cell lysate (30 ug) by using MAFK monoclonal antibody, clone AT2F7 (Cat # MAB11212) (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Enzyme-linked Immunoabsorbent Assay
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Gene Info — MAFK
Entrez GeneID
7975Protein Accession#
NP_002351Gene Name
MAFK
Gene Alias
FLJ32205, MGC71717, NFE2U, P18
Gene Description
v-maf musculoaponeurotic fibrosarcoma oncogene homolog K (avian)
Omim ID
600197Gene Ontology
HyperlinkGene Summary
The developmentally regulated expression of the globin genes depends on upstream regulatory elements termed locus control regions (LCRs). LCRs are associated with powerful enhancer activity that is mediated by the transcription factor NFE2 (nuclear factor erythroid-2). NFE2 recognition sites are also present in the gene promoters of 2 heme biosynthetic enzymes, porphobilinogen deaminase (PBGD; MIM 609806) and ferrochelatase (FECH; MIM 612386). NFE2 DNA-binding activity consists of a heterodimer containing an 18-kD Maf protein (MafF, MafG (MIM 602020), or MafK) and p45 (MIM 601490). Both subunits are members of the activator protein-1 superfamily of basic leucine zipper (bZIP) proteins (see MIM 165160). Maf homodimers suppress transcription at NFE2 sites.[supplied by OMIM
Other Designations
basic-leucine zipper transcription factor MafK|erythroid transcription factor NF-E2 p18 subunit|nuclear factor erythroid-2, ubiquitous (p18)|v-maf avian musculoaponeurotic fibrosarcoma oncogene family, protein K|v-maf musculoaponeurotic fibrosarcoma oncog
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