CISD1 monoclonal antibody, clone AT1A8
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More Files
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Western Blot (Tissue lysate)
Western blot analysis of mouse liver extract (40 ug) by using CISD1 monoclonal antibody, clone AT1A8 (Cat # MAB11208) (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Western Blot (Cell lysate)
Western blot analysis of cell lysates (each 40 ug) by CISD1 monoclonal antibody, clone AT1A8 (Cat # MAB11208) (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1: K562 cell lysate. Lane 2: MCF7 cell lysate.
Flow Cytometry
Flow cytometry analysis of CISD1 in THP-1 cell line, staining at 2-5 ug for 1x106 cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant CISD1.
Immunogen
Recombinant protein corresponding to amino acids 32-108 of human CISD1.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Concentration
1 mg/mL
Isotype
IgG1, kappa
Recommend Usage
ELISA
Flow Cytometry
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of mouse liver extract (40 ug) by using CISD1 monoclonal antibody, clone AT1A8 (Cat # MAB11208) (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Western Blot (Cell lysate)
Western blot analysis of cell lysates (each 40 ug) by CISD1 monoclonal antibody, clone AT1A8 (Cat # MAB11208) (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1: K562 cell lysate. Lane 2: MCF7 cell lysate.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometry analysis of CISD1 in THP-1 cell line, staining at 2-5 ug for 1x106 cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line). -
Gene Info — CISD1
Entrez GeneID
55847Protein Accession#
NP_060934Gene Name
CISD1
Gene Alias
C10orf70, MDS029, MGC14684, ZCD1, mitoNEET
Gene Description
CDGSH iron sulfur domain 1
Gene Ontology
HyperlinkGene Summary
CISD1 is a member of the CDGSH domain-containing family and may play a role in the regulation of mitochondrial oxidative capacity (Wiley et al., 2007, 2007 [PubMed 17376863] [PubMed 17584744]).[supplied by OMIM
Other Designations
OTTHUMP00000019629|zinc finger CDGSH-type domain 1|zinc finger, CDGSH-type domain 1
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Interactome
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Disease
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