ACAT1 monoclonal antibody, clone 2C5
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of cell lysates (each 40 ug) by ACAT1 monoclonal antibody, clone 2C5 (Cat # MAB11206) (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1: HepG2. Lane 2: HeLa.
Immunofluorescence
Immunofluorescence analysis of ACAT1 in Hep3B cells. The cell was stained with ACAT1 monoclonal antibody, clone 2C5 (Cat # MAB11206) (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant ACAT1.
Immunogen
Recombinant protein corresponding to amino acids 34-427 of human ACAT1.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Concentration
1 mg/mL
Isotype
IgG1, kappa
Recommend Usage
ELISA
Immunocytochemistry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of cell lysates (each 40 ug) by ACAT1 monoclonal antibody, clone 2C5 (Cat # MAB11206) (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1: HepG2. Lane 2: HeLa.Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of ACAT1 in Hep3B cells. The cell was stained with ACAT1 monoclonal antibody, clone 2C5 (Cat # MAB11206) (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Enzyme-linked Immunoabsorbent Assay
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Gene Info — ACAT1
Entrez GeneID
38Protein Accession#
NP_000010Gene Name
ACAT1
Gene Alias
ACAT, MAT, T2, THIL
Gene Description
acetyl-Coenzyme A acetyltransferase 1
Gene Ontology
HyperlinkGene Summary
This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. [provided by RefSeq
Other Designations
acetoacetyl Coenzyme A thiolase
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Interactome
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Pathway
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Disease
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