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PFKM monoclonal antibody, clone AT2F11

  • Catalog # : MAB11205
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant PFKM.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 1-780 of human PFKM.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein A purification
  • Concentration:
  • 1 mg/mL
  • Isotype:
  • IgG2a, kappa
  • Recommend Usage:
  • Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Phosphate-Buffered Saline (pH 7.4) containing 10% Glycerol, 0.01% Sodium Azide.
  • Storage Instruction:
  • Store at 4°C. For long term storage, aliquot and store at -20°C. Avoid repeated freezing and thawing cycles.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of HepG2 cell lysate (30 ug) by using PFKM monoclonal antibody, clone AT2F11 (Cat # MAB11205) (1:500-1:2000). Lane1 : 1:500. Lane2 : 1:1000. Lane 3 : 1:2000.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 5213
  • Gene Name:
  • PFKM
  • Gene Alias:
  • GSD7,MGC8699,PFK-1,PFK-M,PFKX
  • Gene Description:
  • phosphofructokinase, muscle
  • Gene Summary:
  • The PFKM gene encodes the muscle isoform of phosphofructokinase (PFK) (ATP:D-fructose-6-phosphate-1-phosphotransferase, EC 2.7.1.11). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. Mammalian PFK is a tetramer made up of various combinations of 3 subunits: muscle (PFKM), liver (PFKL; MIM 171860), and platelet (PFKP; MIM 171840), the genes for which are located on chromosomes 12q13, 21q22, and 10p, respectively. The composition of the tetramers differs according to the tissue type. Muscle and liver PFK are a homotetramers of 4M and 4L subunits, respectively. Erythrocytes contain both L and M subunits, which randomly tetramerize to form M4, L4, and M3L, M2L2, and ML3 hybrid forms of the holoenzyme (Vora et al., 1980 [PubMed 6444721]; Raben and Sherman, 1995 [PubMed 7550225]).[supplied by OMIM
  • Other Designations:
  • phosphofructokinase, muscle type,phosphofructokinase, polypeptide X
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