CNDP2 monoclonal antibody, clone AT15E5
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More Files
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Western Blot (Tissue lysate)
Western blot analysis of mouse kidney extract (40 ug) by using CNDP2 monoclonal antibody, clone AT15E5 (Cat # MAB11202) (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Western Blot
Western blot analysis of lysates (each 40 ug) by CNDP2 monoclonal antibody, clone AT15E5 (Cat # MAB11202) (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1: Mouse liver tissue lysate. Lane 2: HepG2 cell lysate.
Immunofluorescence
Immunofluorescence analysis of CNDP2/CPGL in HeLa cells. The cell was stained with CNDP2 monoclonal antibody, clone AT15E5 (Cat # MAB11202) (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant CNDP2.
Immunogen
Recombinant protein corresponding to amino acids 1-475 of human CNDP2.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Concentration
1 mg/mL
Isotype
IgG1, kappa
Recommend Usage
ELISA
Immunocytochemistry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of mouse kidney extract (40 ug) by using CNDP2 monoclonal antibody, clone AT15E5 (Cat # MAB11202) (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Western Blot
Western blot analysis of lysates (each 40 ug) by CNDP2 monoclonal antibody, clone AT15E5 (Cat # MAB11202) (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1: Mouse liver tissue lysate. Lane 2: HepG2 cell lysate.Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of CNDP2/CPGL in HeLa cells. The cell was stained with CNDP2 monoclonal antibody, clone AT15E5 (Cat # MAB11202) (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Enzyme-linked Immunoabsorbent Assay
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Gene Info — CNDP2
Entrez GeneID
55748Protein Accession#
NP_060705Gene Name
CNDP2
Gene Alias
CN2, CPGL, FLJ10830, HsT2298, PEPA
Gene Description
CNDP dipeptidase 2 (metallopeptidase M20 family)
Omim ID
169800Gene Ontology
HyperlinkGene Summary
CNDP2, also known as tissue carnosinase and peptidase A (EC 3.4.13.18), is a nonspecific dipeptidase rather than a selective carnosinase (Teufel et al., 2003 [PubMed 12473676]).[supplied by OMIM
Other Designations
CNDP dipeptidase 2|cytosolic nonspecific dipeptidase|peptidase A
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Interactome
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Disease
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Publication Reference
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Carnosine's inhibitory effect on glioblastoma cell growth is independent of its cleavage.
Oppermann H, Purcz K, Birkemeyer C, Baran-Schmidt R, Meixensberger J, Gaunitz F.
Amino Acids 2019 May; 51(5):761.
Application：WB, Human, Human glioblastoma cells.
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Carnosine's inhibitory effect on glioblastoma cell growth is independent of its cleavage.
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