SBDS monoclonal antibody, clone AT1E8
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of HeLa cell lysate (40 ug) by using SBDS monoclonal antibody, clone AT1E8 (Cat # MAB11197) (1:3000).
Immunofluorescence
Immunofluorescence analysis of SBDS in HeLa cells. The cell was stained with SBDS monoclonal antibody, clone AT1E8 (Cat # MAB11197) The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant SBDS.
Immunogen
Recombinant protein corresponding to amino acids 1-250 of human SBDS.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Concentration
1 mg/mL
Isotype
IgG2b, kappa
Recommend Usage
ELISA
Immunocytochemistry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of HeLa cell lysate (40 ug) by using SBDS monoclonal antibody, clone AT1E8 (Cat # MAB11197) (1:3000).Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of SBDS in HeLa cells. The cell was stained with SBDS monoclonal antibody, clone AT1E8 (Cat # MAB11197) The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Enzyme-linked Immunoabsorbent Assay
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Gene Info — SBDS
Entrez GeneID
51119Protein Accession#
NP_057122Gene Name
SBDS
Gene Alias
CGI-97, FLJ10917, SDS, SWDS
Gene Description
Shwachman-Bodian-Diamond syndrome
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of a highly conserved protein family that exists from archaea to vertebrates and plants. The encoded protein may function in RNA metabolism. Mutations within this gene are associated with Shwachman-Bodian-Diamond syndrome. An alternative transcript has been described, but its biological nature has not been determined. This gene has a closely linked pseudogene that is distally located. [provided by RefSeq
Other Designations
Shwachman-Bodian-Diamond syndrome protein
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Interactome
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Disease
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