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Last updated: 2020/5/24

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GCLM monoclonal antibody, clone AT2D12 

  • Catalog # : MAB11195
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant GCLM.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 1-274 of human GCLM.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Concentration:
  • 1 mg/mL
  • Isotype:
  • IgG1, kappa
  • Recommend Usage:
  • ELISA
    Immunocytochemistry
    Immunofluorescence
    Western Blot
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
  • Storage Instruction:
  • Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of HeLa cell lysate (40 ug) by using GCLM monoclonal antibody, clone AT2D12 (Cat # MAB11195) (1:3000).
  • Immunocytochemistry
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis of GCLM in HeLa cells. The cell was stained with GCLM monoclonal antibody, clone AT2D12 (Cat # MAB11195) (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
  • Enzyme-linked Immunoabsorbent Assay
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunocytochemistry
  • Enzyme-linked Immunoabsorbent Assay
  • Gene Information
  • Entrez GeneID:
  • 2730
  • Gene Name:
  • GCLM
  • Gene Alias:
  • GLCLR
  • Gene Description:
  • glutamate-cysteine ligase, modifier subunit
  • Gene Summary:
  • Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. [provided by RefSeq
  • Other Designations:
  • GSC light chain,OTTHUMP00000012299,gamma-glutamylcysteine synthetase,glutamate-cysteine ligase (gamma-glutamylcysteine synthetase), regulatory (30.8kD),glutamate-cysteine ligase regulatory protein
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