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KLK3 monoclonal antibody, clone AT1D10 

  • Catalog # : MAB11194
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant KLK3.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 25-261 of human KLK3.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Concentration:
  • 1 mg/mL
  • Isotype:
  • IgG1, kappa
  • Recommend Usage:
  • ELISA
    Flow Cytometry
    Immunocytochemistry
    Immunofluorescence
    Western Blot
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
  • Storage Instruction:
  • Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of PC-3 cell lysate (40 ug) by using KLK3 monoclonal antibody, clone AT1D10 (Cat # MAB11194) (1:500-1:5000). Lane1 : 1:500. Lane2 : 1:1000. Lane 3 : 1:5000.
  • Immunocytochemistry
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis of KLK3 in LNCap cells. The cell was stained with KLK3 monoclonal antibody, clone AT1D10 (Cat # MAB11194) (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
  • Enzyme-linked Immunoabsorbent Assay
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometry analysis of KLK3 in LNCaP cell line, staining at 2-5 ug for 1x106 cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunocytochemistry
  • Enzyme-linked Immunoabsorbent Assay
  • Gene Information
  • Entrez GeneID:
  • 354
  • Protein Accession#:
  • P07288; NP_001639
  • Gene Name:
  • KLK3
  • Gene Alias:
  • APS,KLK2A1,PSA,hK3
  • Gene Description:
  • kallikrein-related peptidase 3
  • Gene Summary:
  • Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its protein product is a protease present in seminal plasma. It is thought to function normally in the liquefaction of seminal coagulum, presumably by hydrolysis of the high molecular mass seminal vesicle protein. Serum level of this protein, called PSA in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. Alternate splicing of this gene generates several transcript variants encoding different isoforms. [provided by RefSeq
  • Other Designations:
  • P-30 antigen,gamma-seminoprotein,kallikrein 3, (prostate specific antigen),prostate specific antigen,semenogelase,seminin
  • Interactome
  • Interactome
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