KRT23 monoclonal antibody, clone AT2F6
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of cell lysates (each 40 ug) by KRT23 monoclonal antibody, clone AT2F6 (Cat # MAB11191) (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1: HeLa cell lysate. Lane 2: HepG2 cell lysate.
Immunofluorescence
Immunofluorescence analysis of KRT23 in Hep3B cells. The cell was stained with KRT23 monoclonal antibody, clone AT2F6 (Cat # MAB11191) (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Flow Cytometry
Flow cytometry analysis of KRT23 in Hep3B cell line, staining at 2-5 ug for 1x106 cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant KRT23.
Immunogen
Recombinant protein corresponding to amino acids 271-422 of human KRT23.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Concentration
1 mg/mL
Isotype
IgG1, kappa
Recommend Usage
ELISA
Flow Cytometry
Immunocytochemistry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of cell lysates (each 40 ug) by KRT23 monoclonal antibody, clone AT2F6 (Cat # MAB11191) (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1: HeLa cell lysate. Lane 2: HepG2 cell lysate.Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of KRT23 in Hep3B cells. The cell was stained with KRT23 monoclonal antibody, clone AT2F6 (Cat # MAB11191) (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometry analysis of KRT23 in Hep3B cell line, staining at 2-5 ug for 1x106 cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. -
Gene Info — KRT23
Entrez GeneID
25984Protein Accession#
NP_056330Gene Name
KRT23
Gene Alias
CK23, DKFZp434G032, HAIK1, K23, MGC26158
Gene Description
keratin 23 (histone deacetylase inducible)
Omim ID
606194Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21. [provided by RefSeq
Other Designations
cytokeratin 23|histone deacetylase inducible keratin 23|hyperacetylation-inducible type I keratin|keratin 23|keratin, type I cytoskeletal 23|type I intermediate filament cytokeratin
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Interactome
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Disease
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