HK2 monoclonal antibody, clone 1A7
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: K562 cell lysate, Lane 2: Jurkat cell lysate.
Western Blot (Recombinant protein)
Western blot analysis of Lane 1: Hexokinase 1 recombinant protein, Lane 2: Hexokinase 2 recombinant protein, Lane 3: Hexokinase 3 recombinant protein, Lane 4: Hexokinase 4 recombinant protein.
Immunofluorescence
Immunofluorescence analysis of Jurkat cells. The cell was stained with HK2 monoclonal antibody, clone 1A7 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Flow Cytometry
Flow cytometric analysis of Jurkat cells. The cell was stained with HK2 monoclonal antibody, clone 1A7 at 2-5 ug for 1x106cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).
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Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant HK2.
Immunogen
Recombinant protein corresponding to full length human HK2.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG1, kappa
Recommend Usage
ELISA
Flow Cytometry
Immunocytochemistry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: K562 cell lysate, Lane 2: Jurkat cell lysate.Western Blot (Recombinant protein)
Western blot analysis of Lane 1: Hexokinase 1 recombinant protein, Lane 2: Hexokinase 2 recombinant protein, Lane 3: Hexokinase 3 recombinant protein, Lane 4: Hexokinase 4 recombinant protein.Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of Jurkat cells. The cell was stained with HK2 monoclonal antibody, clone 1A7 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of Jurkat cells. The cell was stained with HK2 monoclonal antibody, clone 1A7 at 2-5 ug for 1x106cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray). -
Gene Info — HK2
Entrez GeneID
3099GeneBank Accession#
NM_000189Protein Accession#
NP_000180Gene Name
HK2
Gene Alias
DKFZp686M1669, HKII, HXK2
Gene Description
hexokinase 2
Omim ID
601125Gene Ontology
HyperlinkGene Summary
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. [provided by RefSeq
Other Designations
hexokinase-2, muscle
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Interactome
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Pathway
- Amino sugar and nucleotide sugar metabolism
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Fructose and mannose metabolism
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Publication Reference
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Lung ventilation during treadmill locomotion in a semi-aquatic turtle, Trachemys scripta.
Landberg T, Mailhot JD, Brainerd EL.
Journal of Experimental Zoology. Part A, Ecological Genetics and Physiology 2009 Oct; 311(8):551.
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Sequence of human hexokinase III cDNA and assignment of the human hexokinase III gene (HK3) to chromosome band 5q35.2 by fluorescence in situ hybridization.
Furuta H, Nishi S, Le Beau MM, Fernald AA, Yano H, Bell GI.
Genomics 1996 Aug; 36(1):206.
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Lung ventilation during treadmill locomotion in a semi-aquatic turtle, Trachemys scripta.
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