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Last updated: 2020/8/2
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HK2 monoclonal antibody, clone 1A7 

  • Catalog # : MAB1118
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against full length recombinant HK2.
  • Immunogen:
  • Recombinant protein corresponding to full length human HK2.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Isotype:
  • IgG1, kappa
  • Recommend Usage:
  • ELISA
    Flow Cytometry
    Immunocytochemistry
    Immunofluorescence
    Western Blot
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
  • Storage Instruction:
  • Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of Lane 1: K562 cell lysate, Lane 2: Jurkat cell lysate.
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Western blot analysis of Lane 1: Hexokinase 1 recombinant protein, Lane 2: Hexokinase 2 recombinant protein, Lane 3: Hexokinase 3 recombinant protein, Lane 4: Hexokinase 4 recombinant protein.
  • Immunocytochemistry
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis of Jurkat cells. The cell was stained with HK2 monoclonal antibody, clone 1A7 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
  • Enzyme-linked Immunoabsorbent Assay
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometry analysis of Jurkat cells. The cell was stained with HK2 monoclonal antibody, clone 1A7 at 2-5 ug for 1x106cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • Immunocytochemistry
  • Enzyme-linked Immunoabsorbent Assay
  • Gene Information
  • Entrez GeneID:
  • 3099
  • Gene Name:
  • HK2
  • Gene Alias:
  • DKFZp686M1669,HKII,HXK2
  • Gene Description:
  • hexokinase 2
  • Gene Summary:
  • Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. [provided by RefSeq
  • Other Designations:
  • hexokinase-2, muscle
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