PPP2CB monoclonal antibody
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant protein of PPP2CB.
Immunogen
Recombinant protein corresponding to C-terminus of human PPP2CB.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:5000-1:20000)
Western Blot (1:100-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of PPP2CB monoclonal antibody (Cat # MAB11161) at 1:1000 dilution.
Lane 1: A-549 whole cell lysate 40 ug/lane.
Lane 2: A-431 whole cell lysate 40 ug/lane (EGF).
Lane 3: T-47D whole cell lysate 40 ug/lane.
Lane 4: K-562whole cell lysate 40 ug/lane.
Lane 5: HeLa whole cell lysate 40 ug/lane.
Predicted band size: 74 KDa. Observed band size: 74 KDa. Additional band size: 40 KDaEnzyme-linked Immunoabsorbent Assay
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Gene Info — PPP2CB
Entrez GeneID
5516Protein Accession#
P62714;NM_001009552.1Gene Name
PPP2CB
Gene Alias
PP2CB
Gene Description
protein phosphatase 2 (formerly 2A), catalytic subunit, beta isoform
Omim ID
176916Gene Ontology
HyperlinkGene Summary
This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes a beta isoform of the catalytic subunit. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq
Other Designations
PP2A-beta|protein phosphatase 2, catalytic subunit, beta isoform|protein phosphatase type 2A catalytic subunit|serine/threonine protein phosphatase 2A, catalytic subunit, beta isoform
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Interactome
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Pathway
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