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Last updated: 2017/11/19

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PDHA1 monoclonal antibody

  • Catalog # : MAB11156
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant protein of PDHA1.
  • Immunogen:
  • Recombinant protein corresponding to C-terminus of human PDHA1.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Recommend Usage:
  • ELISA (1:5000-1:20000)
    Western Blot (1:100-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4 (1% BSA, 50% glycerol, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of PDHA1 monoclonal antibody (Cat # MAB11156) at 1:1000 dilution.
    Lane1: A-549 whole cell lysate 40 ug/lane.
    Lane 2: HeLa whole cell lysate 40 ug/lane.
    Predicted band size: 43 KDa. Observed band size: 50 KDa.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 5160
  • Protein Accession#:
  • P08559;NM_000284.3
  • Gene Name:
  • PDHA1
  • Gene Alias:
  • PDHA,PDHCE1A,PHE1A
  • Gene Description:
  • pyruvate dehydrogenase (lipoamide) alpha 1
  • Gene Summary:
  • The pyruvate dehydrogenase complex is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA. The PDH complex is composed of multiple copies of 3 enzymes: E1 (PDHA1); dihydrolipoyl transacetylase (DLAT; MIM 608770) (E2; EC 2.3.1.12); and dihydrolipoyl dehydrogenase (DLD; MIM 238331) (E3; EC 1.8.1.4). The E1 enzyme is a heterotetramer of 2 alpha and 2 beta subunits. The E1-alpha subunit contains the E1 active site and plays a key role in the function of the PDH complex (Brown et al., 1994 [PubMed 7853374]).[supplied by OMIM
  • Other Designations:
  • OTTHUMP00000023015,pyruvate dehydrogenase E1 alpha subunit
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