PDHA1 monoclonal antibody
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant protein of PDHA1.
Immunogen
Recombinant protein corresponding to C-terminus of human PDHA1.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:5000-1:20000)
Western Blot (1:100-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of PDHA1 monoclonal antibody (Cat # MAB11156) at 1:1000 dilution.
Lane1: A-549 whole cell lysate 40 ug/lane.
Lane 2: HeLa whole cell lysate 40 ug/lane.
Predicted band size: 43 KDa. Observed band size: 50 KDa.Enzyme-linked Immunoabsorbent Assay
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Gene Info — PDHA1
Entrez GeneID
5160Protein Accession#
P08559;NM_000284.3Gene Name
PDHA1
Gene Alias
PDHA, PDHCE1A, PHE1A
Gene Description
pyruvate dehydrogenase (lipoamide) alpha 1
Gene Ontology
HyperlinkGene Summary
The pyruvate dehydrogenase complex is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA. The PDH complex is composed of multiple copies of 3 enzymes: E1 (PDHA1); dihydrolipoyl transacetylase (DLAT; MIM 608770) (E2; EC 2.3.1.12); and dihydrolipoyl dehydrogenase (DLD; MIM 238331) (E3; EC 1.8.1.4). The E1 enzyme is a heterotetramer of 2 alpha and 2 beta subunits. The E1-alpha subunit contains the E1 active site and plays a key role in the function of the PDH complex (Brown et al., 1994 [PubMed 7853374]).[supplied by OMIM
Other Designations
OTTHUMP00000023015|pyruvate dehydrogenase E1 alpha subunit
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Interactome
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Pathway
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Butanoate metabolism
- Citrate cycle (TCA cycle)
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