H2AFZ monoclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of NIH/3T3 whole cell lysate 40 ug/lane with H2AFZ monoclonal antibody (Cat # MAB11149) at 1:1000 dilution.
Predicted band size: 13 KDa. Observed band size: 17 KDa. Additional band size: 30 KDa.
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of H2AFZ.
Immunogen
A synthetic peptide corresponding to human H2AFZ.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:5000-1:20000)
Western Blot (1:100-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of NIH/3T3 whole cell lysate 40 ug/lane with H2AFZ monoclonal antibody (Cat # MAB11149) at 1:1000 dilution.
Predicted band size: 13 KDa. Observed band size: 17 KDa. Additional band size: 30 KDa.Enzyme-linked Immunoabsorbent Assay
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Gene Info — H2AFZ
Entrez GeneID
3015Protein Accession#
P0C0S5;NM_002106.3Gene Name
H2AFZ
Gene Alias
H2A.z, H2A/z, H2AZ, MGC117173
Gene Description
H2A histone family, member Z
Omim ID
142763Gene Ontology
HyperlinkGene Summary
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent member of the histone H2A family that is distinct from other members of the family. Studies in mice have shown that this particular histone is required for embryonic development and indicate that lack of functional histone H2A leads to embryonic lethality. [provided by RefSeq
Other Designations
H2AZ histone
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Interactome
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Pathway
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