GAPDH monoclonal antibody, clone 1D4
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Specification
Product Description
Mouse monoclonal antibody raised against of GAPDH.
Immunogen
Purified pig GAPDH.
Host
Mouse
Reactivity
Human, Mouse, Rat
Form
Liquid
Recommend Usage
Immunofluorescence (1:100-400)
Western Blot (1:1000-4000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (0.05% sodium azide).
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.
Aliquot will be stable at 4°C for 3 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to opening the cap.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blots analysis of HeLa (1), Jurkat (2) and Daudi (3) cell.
The antibody recognizes a 37 kDa band in a Western Blot analysis.Immunofluorescence
Immunofluorescence staining of SH-SY5Y cells, counter-stained with Hoechst DNA dye.
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