ESR1 monoclonal antibody
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant protein of ESR1.
Immunogen
Recombinant protein corresponding to human ESR1.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:5000-1:20000)
Western Blot (1:100-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
Western blot analysis of recombinant ESR1 protein with a GST tag with ESR1 monoclonal antibody (Cat # MAB11145) at 1:2000 dilution.
Predicted band size: 55 KDa. Observed band size: 55 KDa.Immunocytochemistry
Immunofluorescence
Immunofluorescence
Immunofluorescence staining of HeLa cells using ESR1 monoclonal antibody (Cat # MAB11145) at 1:200 dilution.Enzyme-linked Immunoabsorbent Assay
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Gene Info — ESR1
Entrez GeneID
2099Protein Accession#
P03372;NM_000125.2Gene Name
ESR1
Gene Alias
DKFZp686N23123, ER, ESR, ESRA, Era, NR3A1
Gene Description
estrogen receptor 1
Gene Ontology
HyperlinkGene Summary
This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative splicing results in several transcript variants, which differ in their 5' UTRs and use different promoters. [provided by RefSeq
Other Designations
OTTHUMP00000017718|OTTHUMP00000017719|estrogen receptor alpha
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