CASP8 monoclonal antibody
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of CASP8.
Immunogen
A synthetic peptide corresponding to C-terminus of human CASP8.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:5000-1:20000)
Western Blot (1:100-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of CASP8 monoclonal antibody (Cat # MAB11142) at 1:1000 dilution.
Lane 1: MOLT 4 whole cell lysate 40 ug/lane.
Lane 2: 293 whole cell lysate 40 ug/lane.
Lane 3: Jurkat whole cell lysate 40 ug/lane.
Lane 4: MCF-7 whole cell lysate 40 ug/lane.
Lane 5: HeLa whole cell lysate: 40 ug/lane.
Predicted band size: 55 KDa. Observedband size: 55 KDa.Enzyme-linked Immunoabsorbent Assay
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Gene Info — CASP8
Entrez GeneID
841Protein Accession#
Q14790;NM_001080125.1Gene Name
CASP8
Gene Alias
ALPS2B, CAP4, FLICE, FLJ17672, MACH, MCH5, MGC78473
Gene Description
caspase 8, apoptosis-related cysteine peptidase
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined. [provided by RefSeq
Other Designations
FADD-homologous ICE/CED-3-like protease|MACH-alpha-1/2/3 protein|MACH-beta-1/2/3/4 protein|Mch5 isoform alpha|OTTHUMP00000163718|OTTHUMP00000163719|OTTHUMP00000163720|caspase 8|caspase 8, apoptosis-related cysteine protease|cysteine protease|procaspase-8
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Interactome
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Pathway
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Disease
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