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CASP8 monoclonal antibody

  • Catalog # : MAB11142
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against synthetic peptide of CASP8.
  • Immunogen:
  • A synthetic peptide corresponding to C-terminus of human CASP8.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Recommend Usage:
  • ELISA (1:5000-1:20000)
    Western Blot (1:100-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4 (1% BSA, 50% glycerol, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of CASP8 monoclonal antibody (Cat # MAB11142) at 1:1000 dilution.
    Lane 1: MOLT 4 whole cell lysate 40 ug/lane.
    Lane 2: 293 whole cell lysate 40 ug/lane.
    Lane 3: Jurkat whole cell lysate 40 ug/lane.
    Lane 4: MCF-7 whole cell lysate 40 ug/lane.
    Lane 5: HeLa whole cell lysate: 40 ug/lane.
    Predicted band size: 55 KDa. Observedband size: 55 KDa.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 841
  • Protein Accession#:
  • Q14790;NM_001080125.1
  • Gene Name:
  • CASP8
  • Gene Alias:
  • ALPS2B,CAP4,FLICE,FLJ17672,MACH,MCH5,MGC78473
  • Gene Description:
  • caspase 8, apoptosis-related cysteine peptidase
  • Gene Summary:
  • This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined. [provided by RefSeq
  • Other Designations:
  • FADD-homologous ICE/CED-3-like protease,MACH-alpha-1/2/3 protein,MACH-beta-1/2/3/4 protein,Mch5 isoform alpha,OTTHUMP00000163718,OTTHUMP00000163719,OTTHUMP00000163720,caspase 8,caspase 8, apoptosis-related cysteine protease,cysteine protease,procaspase-8
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