CARM1 monoclonal antibody
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant protein of CARM1.
Immunogen
Recombinant protein corresponding to C-terminus of human CARM1.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:5000-1:20000)
Western Blot (1:100-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of K-562 whole cell lysate 40 ug/lane with CARM1 monoclonal antibody (Cat # MAB11141) at 1:1000 dilution.
Predicted band size: 63 KDa. Observed band size: 63 KDa.Enzyme-linked Immunoabsorbent Assay
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Gene Info — CARM1
Entrez GeneID
10498Protein Accession#
Q86X55;NM_199141.1Gene Name
CARM1
Gene Alias
PRMT4
Gene Description
coactivator-associated arginine methyltransferase 1
Omim ID
603934Gene Ontology
HyperlinkGene Summary
Protein arginine N-methyltransferases, such as CARM1, catalyze the transfer of a methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-adenosyl-L-homocysteine. Protein arginine methylation has been implicated in signal transduction, metabolism of nascent pre-RNA, and transcriptional activation (Frankel et al., 2002 [PubMed 11724789]).[supplied by OMIM
Other Designations
coactivator-associated arginine methyltransferase-1|protein arginine N-methyltransferase 4
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Interactome
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