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Last updated: 2016/12/4
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NT5E monoclonal antibody, clone 2B6

  • Catalog # : MAB1114
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant NT5E.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 27-252 of human NT5E.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG2b, kappa
  • Recommend Usage:
  • Western Blot (1:1000-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4 (0.09% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of cell lysate of human mesenchymal stem cell (MSCs, 30 ug) was resolved by SDS - PAGE , transferred to NC membrane and probed with NT5E monoclonal antibody , clone 2B6 (1 : 1000) (Cat # MAB1114). Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 4907
  • Gene Name:
  • NT5E
  • Gene Alias:
  • CD73,E5NT,NT,NT5,NTE,eN,eNT
  • Gene Description:
  • 5'-nucleotidase, ecto (CD73)
  • Gene Summary:
  • Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase; EC 3.1.3.5) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases (e.g., see MIM 102700, MIM 300300). Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate.[supplied by OMIM
  • Other Designations:
  • 5' nucleotidase (CD73),5' nucleotidase, ecto,OTTHUMP00000016808,OTTHUMP00000040565,Purine 5-Prime-Nucleotidase,ecto-5'-nucleotidase
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