Upper: Neural progenitors were labeled with anti-rat Nestin polyclonal antibody and stained with conjugated donkey anti-goat secondary anti-body (green). Differentiated neurons were labeled with Tubb3 monoclonal antibody, clone TuJ-1 (Cat # MAB11133) (red). Nuclei were stained with DAPI (blue).
Bottom: Tubb3 staining of neuron-specific class III beta Tubulin in differentiated human neural progenitor cells. Cells were stained using goat anti-mouse Alexa Fluor 488 (green) secondary antibody and counter stained with PI (red).