Western blot analysis of cell lysates of HepG2 (45 ug) were resolved by SDS - PAGE , transferred to NC membrane and probed with PPP1CA monoclonal antibody , clone 4G3 (1 : 1000) (Cat # MAB1112). Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system.
The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies in both human and mice suggest that PP1 is an important regulator of cardiac function. Mouse studies also suggest that PP1 functions as a suppressor of learning and memory. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
protein phosphatase 1, catalytic subunit, alpha,serine/threonine protein phosphatase PP1-alpha 1 catalytic subunit