PPP1CA monoclonal antibody, clone 4G3
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of HepG2 cell lysate.
Immunofluorescence
Immunofluorescence analysis of HeLa cells. The cell was stained with PPP1CA monoclonal antibody, clone 4G3 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant PPP1CA.
Immunogen
Recombinant protein corresponding to amino acids 30-299 of human PPP1CA.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG2a, kappa
Recommend Usage
ELISA
Immunocytochemistry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of HepG2 cell lysate.Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of HeLa cells. The cell was stained with PPP1CA monoclonal antibody, clone 4G3 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Enzyme-linked Immunoabsorbent Assay
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Gene Info — PPP1CA
Entrez GeneID
5499GeneBank Accession#
NM_002708Protein Accession#
NP_002699Gene Name
PPP1CA
Gene Alias
MGC15877, MGC1674, PP-1A, PPP1A
Gene Description
protein phosphatase 1, catalytic subunit, alpha isoform
Omim ID
176875Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies in both human and mice suggest that PP1 is an important regulator of cardiac function. Mouse studies also suggest that PP1 functions as a suppressor of learning and memory. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
protein phosphatase 1, catalytic subunit, alpha|serine/threonine protein phosphatase PP1-alpha 1 catalytic subunit
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Interactome
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Pathway
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Publication Reference
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Gene amplification and overexpression of protein phosphatase 1alpha in oral squamous cell carcinoma cell lines.
Hsu LC, Huang X, Seasholtz S, Potter DM, Gollin SM.
Oncogene 2006 Sep; 25(40):5517.
Application：WB, WB-Tr, Human, Oral squamous cell carcinoma cells, UPCI:SCC131-PP1a siRNA, UPCI:SCC103-PP1a siRNA cells.
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Protein phosphatase 1--targeted in many directions.
Cohen PT.
Journal of Cell Science 2002 Jan; 115(Pt 2):241.
Application：WB, Human, Mammalian cells.
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Gene amplification and overexpression of protein phosphatase 1alpha in oral squamous cell carcinoma cell lines.
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