PNMT monoclonal antibody, clone AT1C11
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Transfected lysate)
The cell lysates (40 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with PNMT monoclonal antibody, clone AT1C11 (Cat # MAB11111) (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1: 293T cell lysate Lane 2: PNMT transfected 293T cell lysate
Immunofluorescence
Immunofluorescence analysis of PNMT in Hep3B cells. The cell was stained with PNMT monoclonal antibody, clone AT1C11 (Cat # MAB11111) (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
-
Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant PNMT.
Immunogen
Recombinant protein corresponding to amino acids 1-282 of human PNMT.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Concentration
1 mg/mL
Isotype
IgG2a, kappa
Recommend Usage
ELISA
Immunocytochemistry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Transfected lysate)
The cell lysates (40 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with PNMT monoclonal antibody, clone AT1C11 (Cat # MAB11111) (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1: 293T cell lysate Lane 2: PNMT transfected 293T cell lysateImmunocytochemistry
Immunofluorescence
Immunofluorescence analysis of PNMT in Hep3B cells. The cell was stained with PNMT monoclonal antibody, clone AT1C11 (Cat # MAB11111) (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Enzyme-linked Immunoabsorbent Assay
-
Gene Info — PNMT
Entrez GeneID
5409Protein Accession#
NP_002677Gene Name
PNMT
Gene Alias
MGC34570, PENT, PNMTase
Gene Description
phenylethanolamine N-methyltransferase
Omim ID
171190Gene Ontology
HyperlinkGene Summary
The product of this gene catalyzes the last step of the catecholamine biosynthesis pathway, which methylates norepinephrine to form epinephrine (adrenaline). The enzyme also has beta-carboline 2N-methyltransferase activity. This gene is thought to play a key step in regulating epinephrine production. [provided by RefSeq
Other Designations
OTTHUMP00000164271|noradrenaline N-methyltransferase|phenylethanolamine N-methylase
-
Interactome
-
Pathway
-
Disease
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com