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PNMT monoclonal antibody, clone AT1C11 

  • Catalog # : MAB11111
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against full length recombinant PNMT.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 1-282 of human PNMT.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Concentration:
  • 1 mg/mL
  • Isotype:
  • IgG2a, kappa
  • Recommend Usage:
  • ELISA
    Immunocytochemistry
    Immunofluorescence
    Western Blot
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
  • Storage Instruction:
  • Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • The cell lysates (40 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with PNMT monoclonal antibody, clone AT1C11 (Cat # MAB11111) (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1: 293T cell lysate Lane 2: PNMT transfected 293T cell lysate
  • Immunocytochemistry
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis of PNMT in Hep3B cells. The cell was stained with PNMT monoclonal antibody, clone AT1C11 (Cat # MAB11111) (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
  • Enzyme-linked Immunoabsorbent Assay
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunocytochemistry
  • Enzyme-linked Immunoabsorbent Assay
  • Gene Information
  • Entrez GeneID:
  • 5409
  • Gene Name:
  • PNMT
  • Gene Alias:
  • MGC34570,PENT,PNMTase
  • Gene Description:
  • phenylethanolamine N-methyltransferase
  • Gene Summary:
  • The product of this gene catalyzes the last step of the catecholamine biosynthesis pathway, which methylates norepinephrine to form epinephrine (adrenaline). The enzyme also has beta-carboline 2N-methyltransferase activity. This gene is thought to play a key step in regulating epinephrine production. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000164271,noradrenaline N-methyltransferase,phenylethanolamine N-methylase
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