Jurkat cell lysates (40 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with PNMT monoclonal antibody, clone AT1C11 (Cat # MAB11111) (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
The product of this gene catalyzes the last step of the catecholamine biosynthesis pathway, which methylates norepinephrine to form epinephrine (adrenaline). The enzyme also has beta-carboline 2N-methyltransferase activity. This gene is thought to play a key step in regulating epinephrine production. [provided by RefSeq