PNPO monoclonal antibody, clone AT2C7
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Cell lysate)
Cell extracts of HepG2 (40 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with PNPO monoclonal antibody, clone AT2C7 (Cat # MAB11110). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1: 1:5000
Lane 2: 1:1000
Lane 3: 1:500
-
Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant PNPO.
Immunogen
Recombinant protein corresponding to amino acids 57-261 of human PNPO.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Concentration
1 mg/mL
Isotype
IgG1, kappa
Recommend Usage
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Cell lysate)
Cell extracts of HepG2 (40 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with PNPO monoclonal antibody, clone AT2C7 (Cat # MAB11110). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1: 1:5000
Lane 2: 1:1000
Lane 3: 1:500Enzyme-linked Immunoabsorbent Assay
-
Gene Info — PNPO
Entrez GeneID
55163Protein Accession#
NP_060599Gene Name
PNPO
Gene Alias
FLJ10535, PDXPO
Gene Description
pyridoxamine 5'-phosphate oxidase
Gene Ontology
HyperlinkGene Summary
The enzyme encoded by this gene catalyzes the terminal, rate-limiting step in the synthesis of pyridoxal 5'-phosphate, also known as vitamin B6. Vitamin B6 is a required co-factor for enzymes involved in both homocysteine metabolism and synthesis of neurotransmitters such as catecholamine. Mutations in this gene result in pyridoxamine 5'-phosphate oxidase (PNPO) deficiency, a form of neonatal epileptic encephalopathy. [provided by RefSeq
Other Designations
pyridoxal 5'-phosphate synthase|pyridoxine 5'-phosphate oxidase
-
Interactome
-
Pathway
-
Disease
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com