Cell extracts of HaCaT were resolved by SDS-PAGE, transferred to PVDF membrane and probed with EGFR monoclonal antibody, clone AT2H8 (Cat # MAB11109) (1:250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Immunofluorescence of HaCaT cells stained with Hoechst 3342 (Blue) or phalloidin-TRITC for nucleus or actin staining, respectably. EGFR monoclonal antibody, clone AT2H8 (Cat # MAB11109) (1:250) was stained with alexa 488 (green).
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq
avian erythroblastic leukemia viral (v-erb-b) oncogene homolog,cell growth inhibiting protein 40,cell proliferation-inducing protein 61,epidermal growth factor receptor