293T cell lysates (40 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with PKLR monoclonal antibody, clone AT1E3 (Cat # MAB11108) (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
R-type/L-type pyruvate kinase,pyruvate kinase 1,pyruvate kinase isozyme R/L,pyruvate kinase type L,pyruvate kinase, liver and RBC type,pyruvate kinase, liver and blood cell,red cell/liver pyruvate kinase