PKLR monoclonal antibody, clone AT1E3
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More Files
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Western Blot (Cell lysate)
293T cell lysates (40 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with PKLR monoclonal antibody, clone AT1E3 (Cat # MAB11108) (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant PKLR.
Immunogen
Recombinant protein corresponding to amino acids 47-574 of human PKLR.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Concentration
1 mg/mL
Isotype
IgG1, kappa
Recommend Usage
Western Blot (1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
293T cell lysates (40 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with PKLR monoclonal antibody, clone AT1E3 (Cat # MAB11108) (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Enzyme-linked Immunoabsorbent Assay
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Gene Info — PKLR
Entrez GeneID
5313Protein Accession#
NP_000289Gene Name
PKLR
Gene Alias
PK1, PKL, PKR, PKRL, RPK
Gene Description
pyruvate kinase, liver and RBC
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
R-type/L-type pyruvate kinase|pyruvate kinase 1|pyruvate kinase isozyme R/L|pyruvate kinase type L|pyruvate kinase, liver and RBC type|pyruvate kinase, liver and blood cell|red cell/liver pyruvate kinase
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Interactome
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Pathway
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Disease
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