CD4+ T cells were sorted from mouse splenocytes, then activated with Anti-mouse CD3 and Anti-mouse CD28, followed by culture in Th1-polarizing conditions, and re-stimulation with PMA and Ionomycin. Lysates from control (lane 1) or PMA and Ionomycin-re- stimulated (lane 2) cells were probed with TBX21 monoclonal antibody, clone eBio39D (Cat # MAB11104) at 2 ug/mL, and revealed with Anti-mouse IgG HRP.
This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is the human ortholog of mouse Tbx21/Tbet gene. Studies in mouse show that Tbx21 protein is a Th1 cell-specific transcription factor that controls the expression of the hallmark Th1 cytokine, interferon-gamma (IFNG). Expression of the human ortholog also correlates with IFNG expression in Th1 and natural killer cells, suggesting a role for this gene in initiating Th1 lineage development from naive Th precursor cells. [provided by RefSeq
T-box expressed in T cells,T-cell-specific T-box transcription factor