RAB5A monoclonal antibody, clone 3A4
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of mouse brain tissue lysate.
Immunofluorescence
Immunofluorescence analysis of U87 MG cells. The cell was stained with RAB5A monoclonal antibody, clone 3A4 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
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Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant RAB5A.
Immunogen
Recombinant protein corresponding to full length human RAB5A.
Host
Mouse
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Isotype
IgG2b, kappa
Recommend Usage
ELISA
Immunocytochemistry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of mouse brain tissue lysate.Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of U87 MG cells. The cell was stained with RAB5A monoclonal antibody, clone 3A4 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Enzyme-linked Immunoabsorbent Assay
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Gene Info — RAB5A
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Interactome
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Pathway
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Disease
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Publication Reference
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Editorial: Retinol binding protein 4 and pathogenesis of diabetes.
McTernan PG, Kumar S.
The Journal of Clinical Endocrinology and Metabolism 2007 Jul; 92(7):2430.
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Retinol binding protein 4 expression in humans: relationship to insulin resistance, inflammation, and response to pioglitazone.
Yao-Borengasser A, Varma V, Bodles AM, Rasouli N, Phanavanh B, Lee MJ, Starks T, Kern LM, Spencer HJ 3rd, Rashidi AA, McGehee RE Jr, Fried SK, Kern PA.
The Journal of Clinical Endocrinology and Metabolism 2007 Jul; 92(7):2590.
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Editorial: Retinol binding protein 4 and pathogenesis of diabetes.
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