SNAP25 monoclonal antibody, clone 4E11
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of mouse brain tissue lysate.
Immunofluorescence
Immunofluorescence analysis of U87 MG cells. The cell was stained with SNAP25 monoclonal antibody, clone 4E11 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Flow Cytometry
Flow cytometric analysis of U87 MG cell line, staining at 2-5 ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
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Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant SNAP25.
Immunogen
Recombinant protein corresponding to full length human SNAP25.
Host
Mouse
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Isotype
IgG1, kappa
Recommend Usage
ELISA
Flow Cytometry
Immunocytochemistry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of mouse brain tissue lysate.Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of U87 MG cells. The cell was stained with SNAP25 monoclonal antibody, clone 4E11 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of U87 MG cell line, staining at 2-5 ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line). -
Gene Info — SNAP25
Entrez GeneID
6616GeneBank Accession#
NM_130811Protein Accession#
NP_570824Gene Name
SNAP25
Gene Alias
FLJ23079, RIC-4, RIC4, SEC9, SNAP, SNAP-25, bA416N4.2, dJ1068F16.2
Gene Description
synaptosomal-associated protein, 25kDa
Omim ID
600322Gene Ontology
HyperlinkGene Summary
Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000030267|OTTHUMP00000030268|resistance to inhibitors of cholinesterase 4 homolog|synaptosomal-associated protein 25
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Interactome
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Pathway
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Disease
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Publication Reference
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Expression and function of SNAP-25 as a universal SNARE component in GABAergic neurons.
Tafoya LC, Mameli M, Miyashita T, Guzowski JF, Valenzuela CF, Wilson MC.
Journal of Neuroscience 2006 Jul; 26(30):7826.
Application：IF, IHC, WB, Mouse, Mouse hippocampal neurons, Hippocampus, Thalamus.
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Increased association of synaptosome-associated protein of 25 kDa with syntaxin and vesicle-associated membrane protein following acrosomal exocytosis of sea urchin sperm.
Schulz JR, Sasaki JD, Vacquier VD.
The Journal of Biological Chemistry 1998 Sep; 273(38):24355.
Application：WB-Ce, Sea Urchin, Sperm.
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Expression and function of SNAP-25 as a universal SNARE component in GABAergic neurons.
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