Western blot analysis of mouse brain extracts (20 ug) were resolved by SDS - PAGE , transferred to NC membrane and probed with UCHL1 monoclonal antibody , clone 3D9 (1 : 1000) (Cat # MAB1100). Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system.
Paraffin embedded sections of human nerve tissue were incubated with UCHL1 monoclonal antibody , clone 3D9 (Cat # MAB1100) (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).
The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease