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Last updated: 2016/12/4
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UCHL1 monoclonal antibody, clone 3D9

  • Catalog # : MAB1100
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against full length recombinant UCHL1.
  • Immunogen:
  • Recombinant protein corresponding to full length human UCHL1.
  • Host:
  • Mouse
  • Reactivity:
  • Human, Mouse
  • Form:
  • Liquid
  • Isotype:
  • IgG2a, kappa
  • Recommend Usage:
  • Western Blot (1:1000-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4 (0.09% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • Western blot analysis of mouse brain extracts (20 ug) were resolved by SDS - PAGE , transferred to NC membrane and probed with UCHL1 monoclonal antibody , clone 3D9 (1 : 1000) (Cat # MAB1100). Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Paraffin embedded sections of human nerve tissue were incubated with UCHL1 monoclonal antibody , clone 3D9 (Cat # MAB1100) (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).
  • ELISA
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 7345
  • Gene Name:
  • UCHL1
  • Gene Alias:
  • PARK5,PGP9.5,Uch-L1
  • Gene Description:
  • ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
  • Gene Summary:
  • The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease
  • Other Designations:
  • ubiquitin C-terminal esterase L1,ubiquitin carboxyl-terminal esterase L1,ubiquitin thiolesterase L1
  • Gene Pathway
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