CLU monoclonal antibody, clone 1A11
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of U87 MG cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of human renal cell carcinoma tissue were incubated with CLU monoclonal antibody , clone 1A11 (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).
Immunofluorescence
Immunofluorescence analysis of U87 MG cells. The cell was stained with CLU monoclonal antibody, clone 1A11 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Flow Cytometry
Flow cytometric analysis of U87 MG cell line, staining at 2-5 ug for 1x10 6cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant CLU.
Immunogen
Recombinant protein corresponding to amino acids 1-333 of human CLU.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG1, kappa
Recommend Usage
ELISA
Flow Cytometry
Immunocytochemistry
Immunofluorescence
Immunohistochemistry
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of U87 MG cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of human renal cell carcinoma tissue were incubated with CLU monoclonal antibody , clone 1A11 (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of U87 MG cells. The cell was stained with CLU monoclonal antibody, clone 1A11 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of U87 MG cell line, staining at 2-5 ug for 1x10 6cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. -
Gene Info — CLU
Entrez GeneID
1191GeneBank Accession#
NM_203339Protein Accession#
NP_001822Gene Name
CLU
Gene Alias
AAG4, APOJ, CLI, KUB1, MGC24903, SGP-2, SGP2, SP-40, TRPM-2, TRPM2
Gene Description
clusterin
Omim ID
185430Gene Ontology
HyperlinkGene Summary
SP-40
Other Designations
OTTHUMP00000128311|aging-associated protein 4|apolipoprotein J|clusterin (complement lysis inhibitor, SP-40,40, sulfated glycoprotein 2, testosterone-repressed prostate message 2, apolipoprotein J)|complement lysis inhibitor|complement-associated protein
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Interactome
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Disease
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Publication Reference
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Comparative multidimensional molecular analyses of pediatric diffuse intrinsic pontine glioma reveals distinct molecular subtypes.
Saratsis AM, Kambhampati M, Snyder K, Yadavilli S, Devaney JM, Harmon B, Hall J, Raabe EH, An P, Weingart M, Rood BR, Magge SN, Macdonald TJ, Packer RJ, Nazarian J.
Acta Neuropathologica 2014 Jun; 127(6):881.
Application：IHC, Human, Brain.
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Up-regulation of the clusterin gene after proteotoxic stress: implication of HSF1-HSF2 heterocomplexes.
Loison F, Debure L, Nizard P, le Goff P, Michel D, le Drean Y.
The Biochemical Journal 2006 Apr; 395(1):223.
Application：WB-Tr, Human, U251 cells.
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Overexpression of clusterin in human breast carcinoma.
Redondo M, Villar E, Torres-Munoz J, Tellez T, Morell M, Petito CK.
The American Journal of Pathology 2000 Aug; 157(2):393.
Application：IHC, Human, Human breast carcinoma tissues.
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Apolipoprotein J (clusterin) induces cholesterol export from macrophage-foam cells: a potential anti-atherogenic function?
Gelissen IC, Hochgrebe T, Wilson MR, Easterbrook-Smith SB, Jessup W, Dean RT, Brown AJ.
The Biochemical Journal 1998 Apr; 331 ( Pt 1):231.
Application：WB-Ce, Mouse, Mouse macrophages.
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Comparative multidimensional molecular analyses of pediatric diffuse intrinsic pontine glioma reveals distinct molecular subtypes.
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