VAMP2 monoclonal antibody, clone 3E5
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of mouse brain tissue lysate.
Western Blot (Recombinant protein)
Western blot analysis of Lane 1: Recombinant human VAMP1 protein; Lane 2: Recombinant human VAMP2 protein.
Immunofluorescence
Immunofluorescence analysis of U87 MG cells. The cell was stained with VAMP2 monoclonal antibody, clone 3E5 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant VAMP2.
Immunogen
Recombinant protein corresponding to amino acids 1-89 of human VAMP2.
Host
Mouse
Reactivity
Human, Rat
Form
Liquid
Purification
Protein G purification
Isotype
IgG1, kappa
Recommend Usage
ELISA
Immunocytochemistry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of mouse brain tissue lysate.Western Blot (Recombinant protein)
Western blot analysis of Lane 1: Recombinant human VAMP1 protein; Lane 2: Recombinant human VAMP2 protein.Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of U87 MG cells. The cell was stained with VAMP2 monoclonal antibody, clone 3E5 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Enzyme-linked Immunoabsorbent Assay
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Gene Info — VAMP2
Entrez GeneID
6844GeneBank Accession#
NM_014232Protein Accession#
NP_055047Gene Name
VAMP2
Gene Alias
FLJ11460, SYB2, VAMP-2
Gene Description
vesicle-associated membrane protein 2 (synaptobrevin 2)
Omim ID
185881Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. This gene is thought to participate in neurotransmitter release at a step between docking and fusion. The protein forms a stable complex with syntaxin, synaptosomal-associated protein, 25 kD, and synaptotagmin. It also forms a distinct complex with synaptophysin. It is a likely candidate gene for familial infantile myasthenia (FIMG) because of its map location and because it encodes a synaptic vesicle protein of the type that has been implicated in the pathogenesis of FIMG. [provided by RefSeq
Other Designations
synaptobrevin 2|vesicle associated membrane protein 2
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Interactome
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Pathway
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Disease
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Publication Reference
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Synaptophysin I controls the targeting of VAMP2/synaptobrevin II to synaptic vesicles.
Pennuto M, Bonanomi D, Benfenati F, Valtorta F.
Molecular Biology of the Cell 2003 Dec; 14(12):4909.
Application：WB-Ti, Rat, Rat brains.
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Amisyn, a novel syntaxin-binding protein that may regulate SNARE complex assembly.
Scales SJ, Hesser BA, Masuda ES, Scheller RH.
The Journal of Biological Chemistry 2002 Aug; 277(31):28271.
Application：WB-Ti, Rat, Rat brains.
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VAMP-1 and VAMP-2 gene expression in rat spinal motoneurones: differential regulation after neuronal injury.
Jacobsson G, Piehl F, Meister B.
The European Journal of Neuroscience 1998 Jan; 10(1):301.
Application：IF, Rat, Spinal cord, Sciatic nerves.
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Synaptophysin I controls the targeting of VAMP2/synaptobrevin II to synaptic vesicles.
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