Western blot analysis of recombinant Synaptobrevin 1 , 2 (each 20 ng) and rat brain extracts (20 ug). Lane 1 : Synaptobrevin 1. Lane 2 : Synaptobrevin 2. Lane 3 : Rat brain. To probed with VAMP2 monoclonal antibody , clone 3E5 (1 : 2000) (Cat # MAB1097). Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system.
The protein encoded by this gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. This gene is thought to participate in neurotransmitter release at a step between docking and fusion. The protein forms a stable complex with syntaxin, synaptosomal-associated protein, 25 kD, and synaptotagmin. It also forms a distinct complex with synaptophysin. It is a likely candidate gene for familial infantile myasthenia (FIMG) because of its map location and because it encodes a synaptic vesicle protein of the type that has been implicated in the pathogenesis of FIMG. [provided by RefSeq
synaptobrevin 2,vesicle associated membrane protein 2