Western blot analysis of cell lysates of HeLa (100 ug) were resolved by SDS - PAGE , transferred to PVDF membrane and probed with GPS2 monoclonal antibody , clone 1E2 (1 : 500) (Cat # MAB1096). Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system.
This gene encodes a protein involved in G protein-mitogen-activated protein kinase (MAPK) signaling cascades. When overexpressed in mammalian cells, this gene could potently suppress a RAS- and MAPK-mediated signal and interfere with JNK activity, suggesting that the function of this gene may be signal repression. The encoded protein is an integral subunit of the NCOR1-HDAC3 (nuclear receptor corepressor 1-histone deacetylase 3) complex, and it was shown that the complex inhibits JNK activation through this subunit and thus could potentially provide an alternative mechanism for hormone-mediated antagonism of AP1 (activator protein 1) function. [provided by RefSeq