Western blot analysis of mouse liver extracts (20 ug) were resolved by SDS - PAGE , transferred to PVDF membrane and probed with BHMT monoclonal antibody , clone 3D6 (1 : 1000) (Cat # MB1095). Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system.
This gene encodes a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed. [provided by RefSeq