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Last updated: 2020/5/24
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CRP monoclonal antibody, clone 5A9 

  • Catalog # : MAB1089
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant CRP.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 19-224 of human CRP.
  • Host:
  • Mouse
  • Reactivity:
  • Human, Mouse
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Isotype:
  • IgG2a, kappa
  • Recommend Usage:
  • ELISA
    Flow Cytometry
    Immunocytochemistry
    Immunofluorescence
    Western Blot
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
  • Storage Instruction:
  • Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of cell lysates (each 40 ug). Lane 1 : NIH/3T3. Lane 2 : HepG2. Lane 3 : Jurkat. Lane 4 : HeLa. Lane 5 : SH - SY5Y. To probed CRP monoclonal antibody , clone 5A9 (1 : 1000) (Cat # MAB1089). Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system.
  • Immunocytochemistry
  • Immunocytochemistry
  • Immunofluorescence analysis of HepG2 cells. The cell was stained with CRP monoclonal antibody, clone 5A9 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
  • Immunofluorescence
  • Enzyme-linked Immunoabsorbent Assay
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometry analysis of HepG2 cell line, staining at 2-5 ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunofluorescence
  • Enzyme-linked Immunoabsorbent Assay
  • Gene Information
  • Entrez GeneID:
  • 1401
  • Gene Name:
  • CRP
  • Gene Alias:
  • MGC149895,MGC88244,PTX1
  • Gene Description:
  • C-reactive protein, pentraxin-related
  • Other Designations:
  • OTTHUMP00000033307
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