Western blot analysis of cell lysates (each 40 ug). Lane 1 : NIH/3T3. Lane 2 : HepG2. Lane 3 : Jurkat. Lane 4 : HeLa. Lane 5 : SH - SY5Y. To probed CRP monoclonal antibody , clone 5A9 (1 : 1000) (Cat # MAB1089). Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system.
Immunocytochemistry
Immunofluorescence analysis of HepG2 cells. The cell was stained with CRP monoclonal antibody, clone 5A9 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Immunofluorescence
Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometry analysis of HepG2 cell line, staining at 2-5 ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).