Western blot analysis of cell lysates (each 40 ug). Lane 1 : NIH/3T3. Lane 2 : HepG2. Lane 3 : Jurkat. Lane 4 : HeLa. Lane 5 : SH - SY5Y. To probed CRP monoclonal antibody , clone 5A9 (1 : 1000) (Cat # MAB1089). Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system.