GSK3B monoclonal antibody, clone 1F7
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of HeLa cell lysate.
Immunofluorescence
Immunocytochemistry analysis of HeLa cell lysate. The cell was stained with GSK3B monoclonal antibody, clone 1F7 (Cat#MAB1087) (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant GSK3B.
Immunogen
Recombinant protein corresponding to amino acids 341-420 of human GSK3B.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG1, kappa
Recommend Usage
ELISA
Flow Cytometry
Immunocytochemistry
Immunofluorescence
Western Blot (1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of HeLa cell lysate.Immunocytochemistry
Immunofluorescence
Immunocytochemistry analysis of HeLa cell lysate. The cell was stained with GSK3B monoclonal antibody, clone 1F7 (Cat#MAB1087) (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
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Gene Info — GSK3B
Entrez GeneID
2932GeneBank Accession#
NM_002093Protein Accession#
NP_002084Gene Name
GSK3B
Gene Alias
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Gene Description
glycogen synthase kinase 3 beta
Omim ID
605004Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene
Other Designations
GSK3beta isoform|glycogen synthase kinase-3 beta
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Interactome
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Pathway
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Disease
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Publication Reference
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In vivo regulation of GSK3 phosphorylation by cholinergic and NMDA receptors.
De Sarno P, Bijur GN, Zmijewska AA, Li X, Jope RS.
Neurobiology of Aging 2006 Mar; 27(3):413.
Application：WB-Ti, Mouse, Hippocampus, Cerebral cortex, Striatum.
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Structural basis for recruitment of glycogen synthase kinase 3beta to the axin-APC scaffold complex.
Dajani R, Fraser E, Roe SM, Yeo M, Good VM, Thompson V, Dale TC, Pearl LH.
The EMBO Journal 2003 Feb; 22(3):494.
Application：Added, Recombinant protein.
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Direct, activating interaction between glycogen synthase kinase-3beta and p53 after DNA damage.
Watcharasit P, Bijur GN, Zmijewski JW, Song L, Zmijewska A, Chen X, Johnson GV, Jope RS.
PNAS 2002 Jun; 99(12):7951.
Application：IP-WB, Human, H1299 cells.
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In vivo regulation of GSK3 phosphorylation by cholinergic and NMDA receptors.
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