The monoclonal antibody reacts with the amino terminus of human CCR5, an approximately 45 kDa member of the G protein-coupled receptor (GPCR) superfamily that binds chemokines including MIP-1 alpha , MIP-1 beta and RANTES.
The optimal working dilution should be determined by the end user.
In aqueous buffer (0.09% sodium azide)
Store at 4°C.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Staining of normal human peripheral blood cells with 0.25 ug of mouse IgG1 kappa isotype control purified (open histogram) or 0.25 ug of CCR5 monoclonal antibody, clone eBioT21/8 (Cat # MAB10868) (filled histogram) followed by Anti-mouse IgG biotin and streptavidin PE. Cells in the lymphocyte gate were used for analysis.
This gene encodes a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. This protein is expressed by T cells and macrophages, and is known to be an important co-receptor for macrophage-tropic virus, including HIV, to enter host cells. Defective alleles of this gene have been associated with the HIV infection resistance. The ligands of this receptor include monocyte chemoattractant protein 2 (MCP-2), macrophage inflammatory protein 1 alpha (MIP-1 alpha), macrophage inflammatory protein 1 beta (MIP-1 beta) and regulated on activation normal T expressed and secreted protein (RANTES). Expression of this gene was also detected in a promyeloblastic cell line, suggesting that this protein may play a role in granulocyte lineage proliferation and differentiation. This gene is located at the chemokine receptor gene cluster region. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq