Western blot analysis of human ES cell lysate (20 ug) was resolved by SDS - PAGE , transferred to PVDF membrane and probed with HSP90B1 monoclonal antibody , clone 2H3 (1 : 500). Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system.
Immunofluorescence staining of human ES cell colony with HSP90B1 monoclonal antibody , clone 2H3 (Cat # MAB1085) .
HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90B1 is an endoplasmic reticulum HSP90 protein. Other HSP90 proteins are found in cytosol (see HSP90AA1; MIM 140571) and mitochondria (TRAP1; MIM 606219) (Chen et al., 2005 [PubMed 16269234]).[supplied by OMIM