HSP90B1 monoclonal antibody, clone 2H3
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of human ES cell lysate.
Immunofluorescence
Immunofluorescence staining of human ES cells.
Flow Cytometry
Flow cytometric analysis of U87MG cells;, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant HSP90B1.
Immunogen
Recombinant protein corresponding to amino acids 676-803 of human HSP90B1.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG2a, kappa
Recommend Usage
ELISA
Flow Cytometry
Immunocytochemistry
Immunofluorescence
Western Blot(1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of human ES cell lysate.Immunocytochemistry
Immunofluorescence
Immunofluorescence staining of human ES cells.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of U87MG cells;, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line). -
Gene Info — HSP90B1
Entrez GeneID
7184GeneBank Accession#
NM_003299Protein Accession#
NP_003290Gene Name
HSP90B1
Gene Alias
ECGP, GP96, GRP94, TRA1
Gene Description
heat shock protein 90kDa beta (Grp94), member 1
Omim ID
191175Gene Ontology
HyperlinkGene Summary
HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90B1 is an endoplasmic reticulum HSP90 protein. Other HSP90 proteins are found in cytosol (see HSP90AA1; MIM 140571) and mitochondria (TRAP1; MIM 606219) (Chen et al., 2005 [PubMed 16269234]).[supplied by OMIM
Other Designations
Tumor rejection antigen-1 (gp96)|endothelial cell (HBMEC) glycoprotein|glucose regulated protein, 94 kDa|heat shock protein 90kDa beta, member 1|tumor rejection antigen (gp96) 1
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Interactome
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Pathway
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Disease
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Publication Reference
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The human embryonal carcinoma marker antigen TRA-1-60 is a sialylated keratan sulfate proteoglycan.
Badcock G, Pigott C, Goepel J, Andrews PW.
Cancer Research 1999 Sep; 59(18):4715.
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TRA-1-60: a new serum marker in patients with germ-cell tumors.
Marrink J, Andrews PW, van Brummen PJ, de Jong HJ, Sleijfer DT, Schraffordt Koops H, Oosterhuis JW.
International Journal of Cancer 1991 Sep; 49(3):368.
Application：ELISA, Human, Human serum.
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Three monoclonal antibodies defining distinct differentiation antigens associated with different high molecular weight polypeptides on the surface of human embryonal carcinoma cells.
Andrews PW, Banting G, Damjanov I, Arnaud D, Avner P.
Hybridoma 1984 Dec; 3(4):347.
Application：Flow Cyt, IHC-Fr, RIA, Human, Mouse, 2102Ep cl2A6 cells, NT2/D1 cells, Xenograft tumors from mouse.
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The human embryonal carcinoma marker antigen TRA-1-60 is a sialylated keratan sulfate proteoglycan.
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