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Last updated: 2016/12/4
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NR3C1 monoclonal antibody, clone 6E6

  • Catalog # : MAB10837
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant NR3C1.
  • Immunogen:
  • Recombinant protein corresponding to human NR3C1.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry (1:200-1:1000)
    Immunofluorescence (1:200-1:1000)
    Flow cytometry (1:200-1:400)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascites (0.03% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis using NR3C1 monoclonal antibody, clone 6E6 (Cat # MAB10837) against HeLa cell lysate.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissues using NR3C1 monoclonal antibody, clone 6E6 (Cat # MAB10837) with DAB staining.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis of PC-2 cells using NR3C1 monoclonal antibody, clone 6E6 (Cat # MAB10837) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • ELISA
  • ELISA
  • ELISA measurement of NR3C1 monoclonal antibody, clone 6E6 (Cat # MAB10837).
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of K-562 cells using NR3C1 monoclonal antibody, clone 6E6 (Cat # MAB10837) (green) and negative control (purple).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 2908
  • Gene Name:
  • NR3C1
  • Gene Alias:
  • GCCR,GCR,GR,GRL
  • Gene Description:
  • nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
  • Gene Summary:
  • The protein encoded by this gene is a receptor for glucocorticoids that can act as both a transcription factor and as a regulator of other transcription factors. This protein can also be found in heteromeric cytoplasmic complexes along with heat shock factors and immunophilins. The protein is typically found in the cytoplasm until it binds a ligand, which induces transport into the nucleus. Mutations in this gene are a cause of glucocorticoid resistance, or cortisol, resistance. Alternate splicing, the use of at least three different promoters, and alternate translation initiation sites result in several transcript variants encoding the same protein or different isoforms, but the full-length nature of some variants has not been determined. [provided by RefSeq
  • Other Designations:
  • nuclear receptor subfamily 3, group C, member 1
  • Related Disease
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