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Last updated: 2016/12/11
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NQO1 monoclonal antibody, clone 4D12

  • Catalog # : MAB10835
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant NQO1.
  • Immunogen:
  • Recombinant protein corresponding to human NQO1.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry (1:200-1:1000)
    Flow cytometry (1:200-1:400)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascites (0.03% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis using NQO1 monoclonal antibody, clone 4D12 (Cat # MAB10835) against A-549 (1), SKNES (2), HepG2 (3), MCF-7 (4) and HeLa (5) cell lysate.
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Western blot analysis using NQO1 monoclonal antibody, clone 4D12 (Cat # MAB10835) against human NQO1 (aa: 134-274) recombinant protein. (Expected MW is 41.3 kDa)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of paraffin-embedded human testis (A) and human ovarian cancer (B) tissues using NQO1 monoclonal antibody, clone 4D12 (Cat # MAB10835) with DAB staining.
  • ELISA
  • ELISA
  • ELISA measurement of NQO1 monoclonal antibody, clone 4D12 (Cat # MAB10835).
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of NIH/3T3 cells using NQO1 monoclonal antibody, clone 4D12 (Cat # MAB10835) (green) and negative control (red).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 1728
  • Gene Name:
  • NQO1
  • Gene Alias:
  • DHQU,DIA4,DTD,NMOR1,NMORI,QR1
  • Gene Description:
  • NAD(P)H dehydrogenase, quinone 1
  • Gene Summary:
  • This gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This protein's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimer's disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq
  • Other Designations:
  • DT-diaphorase,NAD(P)H menadione oxidoreductase 1, dioxin-inducible,NAD(P)H:Quinone acceptor oxidoreductase type 1,NAD(P)H:menadione oxidoreductase 1,NAD(P)H:quinone oxireductase,azoreductase,diaphorase (NADH/NADPH) (cytochrome b-5 reductase),diaphorase-4,
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