NGFR monoclonal antibody, clone 2F1C2
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant NGFR.
Immunogen
Recombinant protein corresponding to human NGFR.
Host
Mouse
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis using NGFR monoclonal antibody, clone 2F1C2 (Cat # MAB10831) against NGFR-hIgGFc transfected HEK293 cell lysate.Immunofluorescence
Immunofluorescence analysis of EC cells (rat aortic endothelial cells) using NGFR monoclonal antibody, clone 2F1C2 (Cat # MAB10831) (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of EC cells (rat aortic endothelial cells) using NGFR monoclonal antibody, clone 2F1C2 (Cat # MAB10831) (green) and negative control (purple). -
Gene Info — NGFR
Entrez GeneID
4804Gene Name
NGFR
Gene Alias
CD271, Gp80-LNGFR, TNFRSF16, p75(NTR), p75NTR
Gene Description
nerve growth factor receptor (TNFR superfamily, member 16)
Omim ID
162010Gene Ontology
HyperlinkGene Summary
Nerve growth factor receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain. [provided by RefSeq
Other Designations
low affinity nerve growth factor receptor|low-affinity nerve growth factor receptor|nerve growth factor receptor
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