NFKB1 monoclonal antibody, clone 5D10D11
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant NFKB1.
Immunogen
Recombinant protein corresponding to human NFKB1.
Host
Mouse
Reactivity
Human
Form
Liquid
Isotype
IgG2a
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using NFKB1 monoclonal antibody, clone 5D10D11 (Cat # MAB10830) against K-562 (1), Jurkat (2), A-431 (3), HeLa (4), THP-1 (5) and MCF-7 (6) cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human intima cancer tissues (A) and human bladder cancer tissues (B) using NFKB1 monoclonal antibody, clone 5D10D11 (Cat # MAB10830) with DAB staining.Immunofluorescence
Immunofluorescence analysis of U251 cells using NFKB1 monoclonal antibody, clone 5D10D11 (Cat # MAB10830) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
ELISA measurement of NFKB1 monoclonal antibody, clone 5D10D11 (Cat # MAB10830).Flow Cytometry
Flow cytometric analysis of MCF-7 cells using NFKB1 monoclonal antibody, clone 5D10D11 (Cat # MAB10830) (green) and negative control (purple). -
Gene Info — NFKB1
Entrez GeneID
4790Gene Name
NFKB1
Gene Alias
DKFZp686C01211, EBP-1, KBF1, MGC54151, NF-kappa-B, NFKB-p105, NFKB-p50, p105, p50
Gene Description
nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
Omim ID
164011Gene Ontology
HyperlinkGene Summary
This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
DNA binding factor KBF1|NF-kappabeta|nuclear factor NF-kappa-B p50 subunit|nuclear factor kappa-B DNA binding subunit|nuclear factor kappa-B, subunit 1
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Interactome
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Pathway
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Disease
- Abortion
- Acute Lung Injury
- Adenocarcinoma
- Alcoholism
- Alzheimer disease
- Arthritis
- Asthma
- Atherosclerosis
- Behcet Syndrome
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