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Last updated: 2016/12/4
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CTSD monoclonal antibody, clone 4G2

  • Catalog # : MAB1083
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant CTSD.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 21-412 of human CTSD.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG2b, kappa
  • Recommend Usage:
  • Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4 (0.09% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of cell lysates (each 20 ug). Lane 1 : HepG2. Lane 2 : Hep3B. Lane 3 : MDA - MB - 231. Lane 4 : MCF - 7. To probed with CTSD monoclonal antibody , clone 4G2 (1 : 1000) (Cat # MAB1083). Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and ECL detection system.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Paraffin embedded sections of human breast lobule tissue were incubated with CTSD monoclonal antibody, clone 4G2 (Cat # MAB1083 ; 1 : 50) for 2 hours at room temperature.
    Antigen retrieval was performed in 100 mM sodium citrate buffer and detected using Diaminobenzidine (DAB).
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 1509
  • Gene Name:
  • CTSD
  • Gene Alias:
  • CLN10,CPSD,MGC2311
  • Gene Description:
  • cathepsin D
  • Gene Summary:
  • This gene encodes a lysosomal aspartyl protease composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. This proteinase, which is a member of the peptidase C1 family, has a specificity similar to but narrower than that of pepsin A. Transcription of this gene is initiated from several sites, including one which is a start site for an estrogen-regulated transcript. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease. [provided by RefSeq
  • Other Designations:
  • lysosomal aspartyl peptidase,lysosomal aspartyl protease
  • Gene Pathway
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