CTSD monoclonal antibody, clone 4G2
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: HepG2 cell lysate; Lane 2: Hep3B cell lysate; Lane 3: MDA-MB-231 cell lysate; Lane 4.: MCF7 cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human breast lobule tissue were incubated with CTSD monoclonal antibody, clone 4G2 (Cat# MAB1083) (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant CTSD.
Immunogen
Recombinant protein corresponding to amino acids 21-412 of human CTSD.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG2b, kappa
Recommend Usage
ELISA
Immunohistochemistry
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: HepG2 cell lysate; Lane 2: Hep3B cell lysate; Lane 3: MDA-MB-231 cell lysate; Lane 4.: MCF7 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human breast lobule tissue were incubated with CTSD monoclonal antibody, clone 4G2 (Cat# MAB1083) (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).Enzyme-linked Immunoabsorbent Assay
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Gene Info — CTSD
Entrez GeneID
1509GeneBank Accession#
NM_001909Protein Accession#
NP_001900Gene Name
CTSD
Gene Alias
CLN10, CPSD, MGC2311
Gene Description
cathepsin D
Gene Ontology
HyperlinkGene Summary
This gene encodes a lysosomal aspartyl protease composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. This proteinase, which is a member of the peptidase C1 family, has a specificity similar to but narrower than that of pepsin A. Transcription of this gene is initiated from several sites, including one which is a start site for an estrogen-regulated transcript. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease. [provided by RefSeq
Other Designations
lysosomal aspartyl peptidase|lysosomal aspartyl protease
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Interactome
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Pathway
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Disease
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Publication Reference
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Biological and clinical significance of cathepsin D in breast cancer metastasis.
Garcia M, Platet N, Liaudet E, Laurent V, Derocq D, Brouillet JP, Rochefort H.
Stem Cells 1996 Nov; 14(6):642.
Application：ELISA, IF, IHC, WB-Ce, WB-Ti, Human, Human breast cancer.
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Isolation and sequencing of a cDNA clone encoding rat liver lysosomal cathepsin D and the structure of three forms of mature enzymes.
Fujita H, Tanaka Y, Noguchi Y, Kono A, Himeno M, Kato K.
Biochemical and Biophysical Research Communications 1991 Aug; 179(1):190.
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Biological and clinical significance of cathepsin D in breast cancer metastasis.
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